Pepstatin bụ pentapeptide, onye na-egbochi aspartyl protease inhibitor, nke nwere ike igbochi aspartic protease na acid protease nke microorganisms dị iche iche.Pepstatin bụ nke ụdị Streptomyces na-ezobe ya nke Streptomyces mepụtara.Ọ nwere ike igbochi pepsin, pepsin D na angiotensin na-ewepụta enzyme, ma nwee mmetụta ọgwụgwọ na ọnya afọ, ọbara mgbali elu akụrụ, ọrịa ogbu na nkwonkwo, carrageenan edema na ọrịa ndị ọzọ.
Pepstatin bụ ihe mgbochi siri ike nke aspartyl protease dị ka pepsin, cathepsin D, na renin.Pentapeptide eke a dịpụrụ adịpụ site na actinomycetes bụ kemgbe ọtụtụ afọ ihe na-egbochi renin in vitro.Pepstatin akọwapụtaghị maka renin na ọ naghị amịchakwa ya na mmiri.Ihe nrụpụta nke pepstatin mụbara solubility na nkọwapụta ya maka renin site n'ọtụtụ iwu dị ukwuu.Pepstatin nwere γ amino acid statin pụrụ iche nke nwere ike dochie anya amino acid abụọ ahụ na njikọ ssissile nke mkpụrụ protein ma gbochie mgbawa mkpụrụ n'ihi ntụnyere nhazi ahụ. na ọnọdụ mgbanwe nke peptide bond hydrolysis site aspartyl proteases.
Mgbe a na-ewepụta protein site na mkpụrụ ndụ ndị gbajiri agbaji, enwere ike ịhapụ proteases, nke dị mkpa ka a gbochie ngwa ngwa ka protein ghara imebi.N'ime usoro mmịpụta protein, ọ dị mkpa ịgbakwunye ndị na-egbochi protease iji gbochie proteolysis.Protease inhibitor na-ezo aka n'ụzọ sara mbara na ihe na-ejikọta ụfọdụ otu na etiti nọ n'ọrụ nke mkpụrụ ndụ protease, nke mere na ọrụ protease na-ebelata ma ọ bụ ọbụna na-apụ n'anya, ma ọ dịghị egbochi protein enzyme.Mmetụta nke protease dị iche iche na protein dị iche iche dị iche iche, n'ihi ya, ọ dị mkpa iji dozie ntinye nke protease dị iche iche.N'ihi na solubility nke protease inhibitor n'ime mmiri mmiri dị oke ala, ekwesịrị itinye uche pụrụ iche na onye na-eme ihe na-egbochi protease kwesịrị ịgwakọta ya n'ụzọ zuru ezu na nchekwa iji belata ọdịda nke protease inhibitor.Pepstantin A nwere ike igbochi protease acid dị ka pepsin, angiotensin, collagenase, cathepsin D na chymosin.
Pepstatin A bụ onye na-egbochi cathepsin d na e.Mgbe ejiri nlezianya mee ihe dị iche iche nke pepstatin A sel HEK293 maka awa 24, ahụrụ ngosipụta nke LC3Ⅱ na p62.Nsonaazụ gosiri na pepstatin A nwere ike ịkwalite ngosipụta nke LC3Ⅱ na P62 (P<0.05) n'ụzọ dabere na dose.A na-eji 20μ g / ml pepstatin A na-emeso HEK293 n'oge dị iche iche, na mmetụta nke oge dị iche iche na-egosipụta na nkwupụta nke LC3II na p62.Nsonaazụ gosiri na pepstatin A nwere ike imeziwanye ngosipụta nke LC3II na p62 n'ụzọ dabere na oge.
Anyị bụ ndị na-emepụta polypeptide na China, nwere ahụmahụ tozuru okè na mmepụta polypeptide ọtụtụ afọ.Hangzhou Taijia Biotech Co., Ltd bụ ọkachamara polypeptide akụrụngwa emeputa, nke nwere ike inye iri puku kwuru iri puku polypeptide akụrụngwa na nwekwara ike ahaziri dị ka mkpa.Ogo nke ngwaahịa polypeptide dị mma, ịdị ọcha nwere ike iru 98%, nke ndị ọrụ ghọtara n'ụwa niile. Nnọọ ịkpọtụrụ anyị.
